Biomaterials, Artificial Organs and Tissue Engineering by Larry L Hench

By Larry L Hench

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155 It was not possible hitherto to visualize low concentrations of polysaccharides by using proton density NMR-imaging because of the surrounding free water. But the T1 weighted sequence selected in the study for imaging enhances the contrast of the microcapsule image by suppressing the proton signals of the aqueous surroundings. The use of NMR to study carbohydrate polymers such as agarose163 and alginate164±167 has been shown feasible under various experimental conditions. 169 Shen et al. 170 Their work shows a method for monitoring 300±400 m microcapsules suitable for gene therapy applications under a clinically relevant setting.

Bourget L, Chang TMS. Phenylalanine ammonia-lyase immobilized in 32 26. 27. 28. 29. 30. 31. 32. 33. 34. 35. 36. 37. 38. 39. 40. Artificial cells, cell engineering and therapy semipermeable microcapsules for enzyme replacement in phenylketonuria. FEBS Letters 1985; 180(1):5±8. Bourget L, Chang TMS. Phenylalanine ammonia-lyase immobilized in microcapsules for the depletion of phenylalanine in plasma in phenylketonuric rat model. Biochimica et Biophysica Acta 1986; 883(3):432±438. Bourget L, Chang TMS.

Hybridoma 2005; 24(3):133±140. 87. Hao SG, Su LP, Gu XL, Moyana T, Xiang J. A novel approach to tumor suppression using microencapsulated engineered J558/TNF-alpha cells. Experimental Oncology 2005; 27(1):56±60. 88. Lohr M, Hoffmeyer A, Kroger JC, Freund M, Hain J, Holle A et al. Microencapsulated cell-mediated treatment of inoperable pancreatic carcinoma. Lancet 2001; 357(9268):1591±1592. 89. Read TA, Farhadi M, Bjerkvig R, Olsen BR, Rokstad AM, Huszthy PC et al. Intravital microscopy reveals novel antivascular and antitumor effects of endostatin delivered locally by alginate-encapsulated cells.

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