Applied Virology by Edouard Kurstak (Auth.)

By Edouard Kurstak (Auth.)

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Cox, J. , and Schneider, L. G. (1978). Dev. Biol. Stand. 40, 45-56. , Cox, J. , and Schneider, L. G. (1979). Virology 98, 63-75. , Wiktor, T. , and Varrichio, A. (1983). J. Virol, (in press). Genetic Engineering Letter (1982). 2, 3, and also Business Week (November, 1982). Henderson, W. (1981). 273. Vienna, Austria. Kleid, D. G. (1982). Engineering Foundation Conferences; Biochemical Engineering III. Santa Barbara, California. Kleid, D. , Moore, D. , Grubman, M. , McKercher, P. , Morgan, D. , Robertson, Β.

Coli. (3) A library of resulting plasmids was screened, and individual clones encoding the VP1 protein were identified, sequenced, and cleaved with restriction endonucleases to fragments encoding only desired parts of protein. (4) These cDNA fragments were annealed into expression vector plasmids containing the E. 26 F. Α. Murphy et al. coli tryptophan promoter-operator system. The plasmids were used to transform E. coli organisms, which were then grown in large cultures. (5) Lysates of these cultures were examined for the presence of FMD VP1 protein analogs, and the presence of biologically active proteins was demonstrated.

Gen. Virol. 65, 197. Kleid, D. , Moore, D. , Grubman, M. , McKercher, P. , Morgan, D. , Robertson, B. , and Bachrach, H. L. (1981). Science 214, 1125. Laemmli, U. K. (1970). Nature (London) 227, 680. Mayer, M. , et al. (1957). Immunol. 78, 435. Meloen, R. , Woortmeyer, R. , and van Zaane, D. (1983). J. Gen. Virol. 64, 1193. 3. Critical Antigenic Site on VP1 of Poliovirus 49 Minor, P. D. (1982). J. Gen. Virol. 59, 307. Minor, P. , Schild, G. C , and Wood, J. M. (1980). J. Gen. Virol. 51, 147. Minor, P.

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