By Edouard Kurstak (Auth.)
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Additional info for Applied Virology
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Coli. (3) A library of resulting plasmids was screened, and individual clones encoding the VP1 protein were identified, sequenced, and cleaved with restriction endonucleases to fragments encoding only desired parts of protein. (4) These cDNA fragments were annealed into expression vector plasmids containing the E. 26 F. Α. Murphy et al. coli tryptophan promoter-operator system. The plasmids were used to transform E. coli organisms, which were then grown in large cultures. (5) Lysates of these cultures were examined for the presence of FMD VP1 protein analogs, and the presence of biologically active proteins was demonstrated.
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