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Additional info for A complete retrograde glossary of the Hittite language
Keep at 4°C for no more than 1 wk. 4. 1% Triton in 10% goat serum. 5. Monoclonal stage-specific embryonic antigen (SSEA) 1 antibody (diluted 1/5; Developmental Studies Hybridoma Bank, Iowa City, IA; cat. no. MC-480). cn ←点击进入 20 Billon, Jolicoeur, and Raff 6. Monoclonal antirat nestin antibody (diluted 1/100, Pharmingen, San Diego, CA; cat. no. 556309). 7. Rabbit anti-NG2 chondroitin sulfate proteoglycan antibodies (diluted 1/50; Chemicon, cat. no. AB5320). 8. Monoclonal anti-GC antibody (supernatant, diluted 1/5) (16).
Biol. 177, 43–53. cn ←点击进入 32 Billon, Jolicoeur, and Raff 55 Pringle, N. , Yu, W. , et al. (1996) Determination of neuroepithelial cell fate: 55. induction of the oligodendrocyte lineage by ventral midline cells and sonic hedgehog. Dev. Biol. 177, 30–42. , Porter, J. , et al. (1995) Floor plate and motor neuron induction 56. by different concentrations of the amino-terminal cleavage product of sonic hedgehog autoproteolysis. Cell 81, 445–455. , Hall, A. , et al. (2001) Hedgehog-dependent oligoden57.
On ES cell growth and differentiation, so it is recommended that cells are tested routinely for mycoplasma (see Note 3). To subculture ES cells: 1. 1% gelatin solution by diluting 2% gelatin in PBS, 37°C. Add 5 mL of this solution to a 25-cm2 flask and incubate for about 20 min. 2. During this time, passage the cells. Gently remove medium and wash with 10 mL PBSEDTA. 5 mL trypsin. Return flask to incubator for 5 min. 3. Gently agitate the flask. 5 mL neutralization medium. Pipet up and down several times to make a single-cell suspension and transfer the cells into a universal tube.